Nucleic acid and peptide aptamers methods and protocols pdf

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nucleic acid and peptide aptamers methods and protocols pdf

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After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In Nucleic Acid and Peptide Aptamers: Methods and Protocols , expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types.

Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity

The selected aptamers are capable of specifically binding to different targeting molecules, which is achieved by the three-dimensional structure of aptamers. Aptamers are similar in function to monoclonal antibodies, and therefore, they are also referred to as "chemical antibodies". Due to their high affinity and specificity and low immunogenicity, aptamers are topics of intense interest in today's biological targeting research especially in tumor research. They not only have high potential for clinical advances in tumor targeting detection but also are highly promising as targeted tumor drug carriers for use in tumor therapy. Various experimental studies have shown that aptamer-based diagnostic and therapeutic methods for liver cancer have great potential for application. This paper summarizes the structure, characteristics, and screening methods of aptamers and reviews the recent research progress on nucleic acid aptamers in the targeted diagnosis and treatment of liver cancer. Core tip: For liver cancer, conventional diagnostic methods have low sensitivity, therapeutic drugs have great side effects, and tumor monitoring is difficult, which limit the diagnosis and treatment of liver cancer.

Metrics details. Today, the treatment of bacterial infections is a major challenge, due to growing rate of multidrug-resistant bacteria, complication of treatment and increased healthcare costs. Moreover, new treatments for bacterial infections are limited. Oligonucleotide aptamers are single stranded DNAs or RNAs with target-selective high-affinity feature, which considered as nucleic acid-based affinity ligands, replacing monoclonal antibodies. The aptamer-based systems have been found to be talented tools in the treatment of microbial infections, regarding their promising anti-biofilm and antimicrobial activities; they can reduce or inhibit the effects of bacterial toxins, and inhibit pathogen invasion to immune cell, as well as they can be used in drug delivery systems.

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Therapeutic applications of nucleic acid aptamers in microbial infections

After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In Nucleic Acid and Peptide Aptamers: Methods and Protocols , expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues. Skip to main content Skip to table of contents.

Protocol DOI: Ribosome display is a very effective and powerful technology for screening functional peptides or polypeptides in vitro. In ribosome display, each peptide or polypeptide phenotype links with its corresponding mRNA genotype through a ribosome. This link can be achieved by the absence of a stop codon in the mRNA, therefore stalling the ribosome at the end of translation with the nascent random sequence peptide extended by a spacer outside of the ribosome tunnel. In this chapter, we describe a method for the use of a further stabilized peptide—ribosome—mRNA complex for ribosome display. In vitro selection, Peptide library, Peptide aptamer. Antibody Data Search Beta.

Therapeutic applications of nucleic acid aptamers in microbial infections

Aptamers from the Latin aptus — fit, and Greek meros — part are oligonucleotide or peptide molecules that bind to a specific target molecule. Aptamers are usually created by selecting them from a large random sequence pool, but natural aptamers also exist in riboswitches. Aptamers can be used for both basic research and clinical purposes as macromolecular drugs.

Baby Santosh, Pramod K. Aptamers are short sequences of nucleic acid DNA or RNA or peptide molecules which adopt a conformation and bind cognate ligands with high affinity and specificity in a manner akin to antibody-antigen interactions. It has been globally acknowledged that aptamers promise a plethora of diagnostic and therapeutic applications.

Nucleic Acid and Peptide Aptamers

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. This approach is based on the theoretical background presented here, in which under the co-presence of two similar targets, a specific binding type can be enriched more than a nonspecifically binding one during repetitive steps of partitioning with no PCR amplification between them. This principle was experimentally confirmed by the selection experiment for influenza virus subtype-specific DNA aptamers.

Aptamers are structured nucleic acid molecules that can bind to their targets with high affinity and specificity. Thus, to address these questions, this perspective is intended to suggest some approaches and tips along with novel selection methods to enhance evolution of aptamers. The advantages and limitations of individual aptamer selection methods and post-SELEX optimizations, along with rational approaches to overcome these limitations are elucidated in each case. Further the impact of chosen selection milieus, linker-systems, aptamer cocktails and detection modules utilized in conjunction with target-specific aptamers, on the overall assay performance are discussed in detail, each with its own advantages and limitations. Finally, success studies of established aptamer-based assays are discussed, highlighting how they utilized some of the suggested methodologies to develop commercially successful point-of-care diagnostic assays. Aptamers recognize and bind to their targets through 3-dimensional shapes and various physiochemical interactions, similar to antibody binding mechanisms.

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Aptamer-based therapeutics and their potential in radiopharmaceutical design. Catia S. Aptamers, short, single stranded oligonucleotide entities, have been developed in the past 15 years against a plethora of targets and for a variety of applications. These range from inhibition of receptors and enzymes to the identification of small molecules in sensor applications, and from the development of targeted therapeutic to the design of novel diagnostic and imaging agents. Furthermore, aptamers have been designed for targets that cover a wide range of diseases, from HIV to tropical diseases, cancer and inflammation. Their easy development and flexibility of use and manipulation, offers further potential. In this paper we review their selection and consider some of the recent applications of aptamers in the design of radiopharmaceuticals for the targeted radiotherapy and medical imaging of disease.

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Nucleic Acid Aptamers: Research Tools in Disease Diagnostics and Therapeutics

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  • In "Nucleic Acid and Peptide Aptamers: Methods and Protocols", ISBN ​; Digitally watermarked, DRM-free; Included format: PDF, EPUB. Valderrama M. - 21.05.2021 at 02:00
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  • Nucleic Acid and Peptide Aptamers. Methods and Protocols PDF · Nucleic Acid Pool Preparation and Characterization. Shawn K. Piasecki, Bradley Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography. Charms22 - 25.05.2021 at 18:24

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